Jornal de Ciências Agrícolas e Pesquisa de Alimentos
Acesso livre

Abstrato

Application of TILLING and Eco TILLING in Crop Improvement

Asmamaw Amogne Mekonen*, Gebeyaw Achenef

Molecular breeding in Ethiopia is in a very gradual introduction process. Beyond the detail understanding and application a technique or procedure factors, establishment cost is a paramount headache. Though, these factors limit its application in Ethiopia context different molecular breeding techniques such as TILLING and Eco TILLING are coming front especially at a university levels in theoretical basis. Targeting induced local lesions in genomes) is a broad reverse-genetic strategy for locating an allelic series of induced point mutations in genes of interest. TILLING allows for the rapid and cost-effective detection of induced point mutations in chemically mutagenized populations. The technique is applicable not only to model organisms but also to economically important plant organisms. TILLING provides a powerful approach for gene discovery, DNA polymorphism assessment, and plant improvement due to several advantages such as simple procedure, high sensitivity, and high efficiency. TILLING and EcoTILLING, when combined with other genomic resources, can be used immediately as a haplotyping tool in plant breeding for identifying allelic variation in genes exhibiting expression correlating with phenotypes and establishing. Thus, this review was examined in 2018 at Bahir Dar college of agriculture and environmental science to create understanding on modern crop improvement methods rather than conventional breeding in this era. Keywords: Eco TILLING; Induced mutation; Nucleotide sequence; TILLING