Jornal de Imunologia Clínica e Celular
Acesso livre

Abstrato

Expression Characteristics of Surface Markers of Memory T cells, CD45RO, CCR7 and CD62L, in Tumor-infiltrating Lymphocytes in Liver Cancer Tissues of Patients with Hepatocellular Carcinomas

Yong Xie, Bi-Wei Luo, Xiao-Dong Yuan, Pei-Kai Tian, Xi Ou, Ze-Wei Lin, Xiao-Ping Liu and Ji-Kui Liu

Purposes: To investigate expression of surface markers on memory T cells and distribution of T cell subsets among tumor-infiltrating lymphocytes (TILs) in liver of hepatocellular-carcinoma (HCC) patients.
Methods: TILs from liver tumor and paracancerous tissue (PT) of 30 HCC patients and 30 subjects who were performed hepatectomy due to benign pathogenic lesion (control) were stained with anti-CD3, CD4, CD8, CD45RO, CD62L and CCR7 antibodies. The distribution of four T cell subsets, CD45RO⁺CD62L⁺CCR7⁺T (central memory T cells, T_CM), CD45RO⁺CD62L^-CCR7^-T (effector memory T cells, TEM), CD45RO⁺ CD62L⁺CCR7^-T (CCR7-T) and CD45RO⁺ CD62L^-CCR7⁺T (CCR7⁺T), among TILs was measured with polychromatic flow cytometry and analyzed statistically.
Results: (1) CD4⁺T_CM subset in three groups accounted for <0.4% of total CD4⁺CD45RO⁺Tm. CD4⁺T_EM subset% in HCC, PT and control was 89.73%, 98.68% and 95.45% of CD4⁺CD45RO⁺Tm,which was significantly lower in HCC than in PT and control. CCR7-T subset% in HCC, PT and control was 0.04, 0.46 and 2.44% of CD4⁺CD45RO⁺Tm, which was significantly lower in HCC and PT than in control. CCR7⁺T subset% in HCC, PT and control group was 9.97%, 0.57% and 1.74% of CD4⁺CD45RO⁺Tm, which was significantly higher in HCC than in PT and control. (2) CD8⁺TCM subset% in HCC, PT and control was 0.41%, 0.55% and 0.26% of CD8⁺CD45RO⁺Tm, which was significantly higher in HCC and PT than in control. CD8+TEM subset% in HCC, PT and control was 92.39%, 98.14% and 98.16% of CD8⁺CD45RO⁺Tm, which was significantly lower in HCC than in PT and control. CCR7-T subset% in HCC, PT and control was 6.44%, 1.03% and 0.75 % of CD8⁺CD45RO⁺Tm which was significantly higher in HCC than in PT and control.
Conclusion: T_EM was the major subset among CD45RO⁺Tm of HCC patients. CD4⁺TEM, CD8⁺T_EM and CCR7^-T subsets were significantly lower whereas CCR7⁺T and CCR7⁺T subsets were significantly higher in tumor than in non-tumor tissues.