Journal of Food: Microbiologia, Segurança e Higiene
Acesso livre

Abstrato

Rapid Detection of Cronobacter and Salmonella in Powdered Infant Formula and Related Matrices Using Loop-Mediated Isothermal Amplification (LAMP)-Bioluminescent Assays as Compared to the Guobiao Standards (GB) Method.

Yan Huang, Raj Rajagopal*, Jianwei Huo, Zhiyong Dai, Can Yi, Jun Zhou, Chenyan Niu, Chenyan Niu , Feng Liu, Qing Tao , Xiqing Wang.

Cronobacter and Salmonella have emerged as major pathogens of concern in Powdered Infant Formula (PIF). China’s PIF industry is growing, and consumers are concerned about the potential risk of these pathogens in PIF. The PIF producers need rapid, easy to use and specific detection of Cronobacter and Salmonella for monitoring of raw materials, process environment and finished products for implementing effective control measures to prevent contamination. The objective of this study was to determine the specificity and sensitivity of LAMP assays compared to the Chinese National Food Safety (Guobiao, GB) Standards 4789.4-2016 (Salmonella) and GB 4789.40-2016 (Cronobacter) detection in raw materials (milk powder, whey powder, minerals, lutein, nucleotide, arachidonic acid powder, docasahexaenoic acid), process environment samples and finished products (neonate and infant PIF). The respective LAMP assays detected Cronobacter and Salmonella in artificially contaminated samples and the results of the LAMP assays were comparable to the respective GB method. The Probability of Detection (POD) analysis showed no significant differences (95% confidence interval) among all the samples tested for both LAMP assays and the respective GB method. The respective LAMP assays enabled rapid detection of Cronobacter and Salmonella in PIF matrices providing next-day results as compared to 3 to 5 days for the GB method.