Jornal de Imunologia Clínica e Celular
Acesso livre

Abstrato

Antitumor Activity of Polysaccharides and Saponin Extracted from Sea Cucumber

Xiurong Su, Caiyun Xu, Yanyan Li, Xiang Gao, Yanru Lou and Jinfeng Ding

The density gradient of S180 cells were treated with various extracts at a concentration of polysaccharides (P1 and P2) and saponins (S1 and S2) measured after 22h, 44h, 68h and 92 h post-treatment. P1, P2, and saponin S1 were extracted from Acaudina leucoproct, and S2 was extracted from the concentrated liquid of Stichopus japonica. The cytotoxic effects of S1 and S2 on S180 cells was demons treated dose and time dependent, using MTT assay. Saponin S2 exhibited the highest potency against S180 cells, with an IC₅₀ of 41.04 µg/ml at 44 h. Additionally, by Annexin V/PI staining, we observed more viable cells under polysaccharides treatment compared to the saponins. These results indicate that the in vitro anti-tumor effects of saponins S1 and S2 are more potent than those of polysaccharides P1 and P2.