Jornal de Cromatografia e Técnicas de Separação
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Comprehensive Two-Dimensional Liquid Chromatography in Metabolome Analysis

Lucas Willmann, Manuel Schlimpert, Daqiang Pan, Christoph Bauer, Jens Trafkowski, Sonja Krieger, Michael Rodamer, Thalia Erbes, Hans Neubauer and Bernd Kammerer

Metabolomics deals with analysis of highly complex biological samples resulting in insufficient separation of relevant metabolites from matrix substances with one-dimensional liquid chromatography. Comprehensive twodimensional liquid chromatography (2D-LC) is a promising technique for the analysis of complex samples because of its increased peak capacity. Comprehensive 2D-LC-MS/MS has been applied for a targeted metabolomics approach regarding metabolites from RNA catabolism. Combination of a Zorbax Eclipse Plus C18 column with a Zorbax Bonus-RP column resulted in increased peak capacity, minimisation of matrix effects and excellent reproducibility.