Medicina Interna: Acesso Aberto
Acesso livre

Abstrato

Co-regulated Intestinal c-kit by Interleukin-17 and Interleukin-10 on Post-infectious Irritable Bowel Syndrome in Mice

Bo Yang, Xuesong Bai, Yijun Yu, Honglan Liu, Cheng Lan, Dan Liu, Xuchun Zhou

Purpose

The aim of this study was to investigate the probable immune mechanisms of PI-IBS.

Methods

C57L/B6 mice, which have been infected by 500 Trichinella larvae for 56 days, were used to PI-IBS model mice, the levels of the intestinal mRNAs and proteins of c-kit, interleukin-10 (IL-10), and IL-17 were measured by RTPCR and western blotting or ELISA. The locations of c-kit were detected using immunohistochemical staining.

Results

Levels of the c-kit protein and mRNA were up-regulated in the whole PI-IBS mice intestine. Compared to the control bowel segments, IL-17 levels were significantly higher in the duodenum and ileum, and IL-10 levels were lower in jejunum, ileum, and colon. By immunohistochemical staining, the increased signals of the c-kit were mainly detected in the submucosa and myenteron.

Conclusions

These results suggested that increased levels of the c-kit resulted in the changes of the intestinal motility and visceral sensitivity in PI-IBS mouse model induced by Trichinella Spiralis infection, and which may be coregulated by IL-17 and IL-10 in pathophysiology of PI-IBS.