Bioquímica e Farmacologia: Acesso Aberto
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Design Self-Assembling Peptide DSAP-2 for 3d Cell Culture and Rapid Hemostasis

Mengmeng Li, Xiaofan Xu, Ran Wang, Duanhua Li, Yuelin Sun, Nan Liu, Yuanyuan Yue, Tianxin Zhao, Jianping Gong, Kanran Wang, Xinyuan Li, Mao Tan, Shanshan Zhang, Kunyue Tan, Zhenyin Chen, Huinan Zhang, Feng Li, Lifeng Jin and Zhongli Luo

Traditional self-assembling peptide can form nanofiber scaffolds to meet the challenges of advance biomaterial, cell culture, tissue engineering and regeneration. L-amino acids have been widely used instead of D-amino acids to design nanomaterial since some D-amino acids have toxicity of cells. Here we report that using D-amino acids to design a new D-form self-assembling peptide DSAP-2 and the circular dichroism, atomic force microscopy and scanning electron microscopy show that the peptide can form nanofiber scaffold as well. Furthermore, cell inhibition assay confirmed this D-form peptide show no toxicity of cells that can support cell growth. Fluorescence microscopy results show that cells had less cell apoptosis in the 3D environment and displayed a fast proliferation after cultured for 7days. Peptide’s hydrogel not only formed nano-scaffolds surrounded by cells in a 3-D cell culture, but achieved rapid hemostasis in a rabbit liver wound model. Our study suggests this peptide could be used in the wound and beyond in the future. This work could also inspire us to design more novel D-form self-assembling peptide in biomaterials and biomedical areas.