Jornal de Cromatografia e Técnicas de Separação

Jornal de Cromatografia e Técnicas de Separação
Acesso livre

ISSN: 2157-7064


Flexible Electrospun Cellulose Fibers as an Affinity Packing Material for the Separation of Bovine Serum Albumin

Minoru Miyauchi, Jianjun Miao, Trevor J. Simmons, Jonathan S. Dordick and Robert J. Linhardt

Flexible, well-dispersed and continuous 100-nm diameter cellulose fibers were prepared from an ionic liquid solvent by a novel dry-jet wet-electro spinning process. The ribbon fibers formed were chemically activated and an affinity dye, cibacron blue (CB) was immobilized at 0.22 g CB/g dry fibers loading to the surface of these fibers. The resulting affinity matrix was packed into a chromatography column and the adsorption, desorption and specificity of this matrix for bovine serum albumin (BSA) was studied. These electrospun fibers had a BSA binding capacity of 230 mg/g, nearly twice that of CB-immobilized 100-?m beads and over ten-fold higher capacity that CB-immobilized cellulose fibers prepared by a conventional electrospinning process. The results of this work suggest that chromatography supports of flexible, well-dispersed, continuous nanofibers may offer advantages over conventional supports in affinity separations.