Espectrometria de Massa e Técnicas de Purificação
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Hydrophilic Interaction Liquid Chromatography for LC-MS

Toshihiko Hanai

The retention mechanisms in hydrophilic interaction liquid chromatography (HILIC) are quantitatively described in in silico analysis. Among a variety of polar bonded-phase silica gels, hexylamine-bonded silica gel is stable and can be used in both aqueous HILIC and ion-exchange liquid chromatography with high reproducibility. Hexynyl and phenylhexyl bonded silica gels are also used in HILIC with high reproducibility. The retention mechanisms in aqueous hydrophilic and non-aqueous hydrophilic (normal-phase) liquid chromatography are similar. Both hydrogenbonding and weak electrostatic interaction contributes to retention. Furthermore, strong electrostatic interaction is dominant in ion-exchange liquid chromatography. A suitable liquid chromatographic system for liquid chromatography (LC)-mass spectrophotometer (MS) is proposed.