Espectrometria de Massa e Técnicas de Purificação
Acesso livre

Abstrato

Important Methods of Mass Spectrometry in Structural and Molecular Biology

Jorg Phan

Mass Spectrometry strategies are used in an expanding number of structural and molecular biology that focus on a better understanding of large biomolecular assemblies. A determination of these investigations is introduced in, outlining that biomolecular building from every single cell compartment and various realms of life have been effectively examined by mass spectrometry. On a very basic level, these methodologies can be partitioned into peptide‐centric and protein‐centric procedures. Peptide-centric techniques involve surface naming methodologies, like hydrogen deuterium exchange mass spectrometry and covalent labeling‐ mass spectrometry, just as cross‐linking‐ mass spectrometry and restricted proteolysis mass spectrometry. These techniques test biomolecular structures in arrangement, using the recently portrayed bottom‐up proteomics work process to identify, at the peptide level, the consequences of the in‐solution try.