Medicina Translacional
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Novel Biomarkers Detection and Identification by Microfluidic- Based MicroELISA

Montaha Lakkis, Junhai Kai, Nelson Santiago, Aniruddha Puntambekar,Victor Moore, Se Hwan Lee, David W Sehy, Ron Schultheis, Jungyoup Han and Chong H Ahn

Miniaturized, high throughput detection technologies including microfluidics systems represent powerful tools for biomarker discovery and analysis. Optimiser™ microplate technology combines microfluidics technology with standard SBS-configured 96-well microplate architecture and allows for the improvement of ELISA workflows. In this review, we present the “standard” and “high sensitivity” capabilities of the Optimiser™ ELISA in detecting human cytokine biomarker; IL-4, resulting in improving sensitivity by 1000-fold higher than the typical conventional ELISA. The Optimiser™ ELISA microplate employs standard ELISA equipment and protocols, selectively reduces sample volume 10-20 fold (Static Mode) and has the capability to amplify assay sensitivity by 1,000 fold (Repetitive Loading Flow-Through Mode) relative to conventional high sensitivity ELISA approaches. Optimiser™ allows sensitive and quantitative detection of biomarkers with demonstrated reproducibility, speed and linearity. In this study we demonstrate the utilization of OptmiserTM in the amplification of low-concentration markers. Optimiser™ microfluidic-based technology represents a revolutionary advancement in ELISA technology, and holds great promise for accurate, sensitive detection of novel biomarkers and its potential applications in clinical diagnosis.