Pediatria Clínica: Acesso Aberto
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Study on Hyperandrogenemia and Insulin Resistance in Children with Idiopathic Central Precocious Puberty Based on Gut Microbiota

Ji Han, Congfu Huang, Junru Chen, Shufen Chen, Bin Wu, Dongming Meng

Object: To study how Gut Microbiota (GM) in Idiopathic Central Precocious Puberty (ICPP) children affects Hyperandrogenemia (HA) and Insulin Resistance (IR).

Methods: In this study, we have recruited 27 ICPP (ICPP group) and 23 healthy children (healthy group) and collected the blood and fecal samples from the participants. Blood samples were tested for hormones, including the follicle-stimulating hormone, luteinizing hormone, estradiol, prolactin and testosterone. Deoxyribonucleic Acid (DNA) was extracted from fecal samples and amplified and sequenced with 16S Ribosomal DNA (16S rDNA) Variable (V3-V4) region. Finally, we annotated the sequencing results, counted the differences in hormone indicators and GM composition between the two groups and analysed the correlation with clinical indicators.

Results: 1. Compared with the healthy group, hormone levels such as Androstenedione (A2), insulin, insulin resistance index and Insulin-like Growth Factor 1 (IGF-1) in the ICPP group were significantly higher (p<0.05); 2. At the phylum level, the ICPP group showed significantly enriched Proteobacteria than the healthy group (4.85% vs. 2.92%); 3. At the genus level, the abundances of Roseburia and Prevotella were significantly higher in the ICPP group than those in the healthy group (7.55% vs. 2.01%, 3.95% vs. 0.19%), but Bacteroides were obviously decreased in the ICPP group (29.96% vs. 44.91%).

Conclusion: Changes in GM promote HA and IR, the latter may be an important pathogenesis in ICPP children.