Engenharia Enzimática
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Comparative Study for Production of Biofuel from Potato Peel Waste as Feedstock by Different Enzymes

Minal Deshmukh, Ashwini Pande

Biofuels are gaining popularity as a viable alternative to petroleum-based energy. Second and third-generation advanced biofuels, which can be made from renewable materials like agricultural waste, appear to be more competitive. Even though there was a minimum concentration of reducing sugars and high-value waste in the existing solutions. Therefore, the proposed research focused on the bioethanol feedstock potential of Potato Peel Waste (PPW), a zerovalue waste. Where, starch, cellulose, hemicellulose, and fermentable sugars are all present in sufficient amounts in PPW. On the other hand, bioethanol made from potato peel waste has a lot of market potential. Consequently, the policy goal is to ensure that a minimum amount of biofuel is readily available in the market at all times to meet demand. To yield large quantities of reducing sugars, a combination of enzymatic treatment with amylase production by Bacillus sp. UEB-S and Amyl glucosidase is used. For effective hydrolysis of PPW liquefaction, termamyl 120 L was utilised, and amyloglucosidase was used for saccharification. Furthermore, hydrolysis of starch from fresh potato tubers by HCl and H₂SO₄ is used to boost the activity of hydrogen ions participating in the reaction as catalyst. Hence, H₂SO₄ was more efficient to hydrolyse potato peel than HCl. Also, the supernatants were tested for reducing sugar using the 3,5-dinitrosalicylic acid (DNS) technique. As a result, the production of biofuel from potato peel waste as feedstock by different enzymes achieves commercial bioethanol production at the lowest possible cost, also reducing waste by-products.