Jornal de Bioquímica e Fisiologia Vegetal
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Effect of Culture Media on Secondary Metabolites from Aspergillus Terrus from Soil of Osun Osogbo Grove

Afolabi-Balogun N.B, Balogun T.K, Oni-Babatunde A.O, Salihu A.K.1, Oyebode N.O., Mosobalaje M.O., Diekola, U.O, T.O Kelani., M. Bashir., Tijani A.I, Lawal A.O, Ganiyu O.T

Fungi belonging to the families; Hypocreaceae, Ascomycota and Basidiomycota are known to produce different secondary metabolite, that are biologically active and have been found to play significant beneficial roles to man. However, the culturing condition as well as the constituent of the media plays significant roles on the quantity and diversity of metabolites produced. In this study, validated pure Aspergillus terrus characterized from the soil of Osun Osogbo Grove was conditioned to produce secondary metabolite using four (4) secondary metabolite induction media: Wickerham medium (WSP); Modified Wickerham medium (MoWSP); 3% Malt Extract broth (MEB) and Tubaki in a 250ml conical flask. Purified A. terrus inoculum was cultured in flasks containing 100ml culture media of varying compositions, u same physiological culturing parameters. Cultures were incubated in a stationary position in the dark for twenty-one (21) days prior extraction of extracellular (Mycelium) and intracellular (Broth) metabolites. Physical evaluation of culture revealed the morphology of organism grown on MEB, WSP and MoWSP has similar fungal growth pattern while Tubaki produce fungal with different morphological pattern. Gas Chromatography Mass Spectrophotometer (GC-MS) analysis of intracellular extract revealed eight (8) metabolites were consistent in all three media while Furo [2,3-c] pyridine, 2,3-dihydro-2,7-dimethyl- was also consistent in the extracellular extract of the three 0media. Three nucleotide analogues; {1,2,4} Triazolo {1,5-a} pyrimidine-6-carboxylic acid,7-amino ethyl esters, 1,2,4}Triazolo{1,5-a}pyrimidine-6-carboxylic acid,7-imino ethyl esters and 2,3-dihydro-2,7-dimethylFuro[2.3-c]pyridine were detected. Structural and functional elucidation of secondary metabolites from MEB revealed A. terrus is a potential novel source for anticancer, immunosuppressor, anti-helminths, antioxidant, antibiotics as well as pesticide.