Jornal de Pesquisa e Desenvolvimento
Acesso livre

Abstrato

Effect of Ubiquinone and Resveratrol on Experimentally Induced Parkinsonism

Ahmed M Kabel and Sanad S El Kholy

Parkinson's disease is one of the motor system diseases caused by factors that interfere with survival of the dopaminergic neurons in the substantia nigra. Its mechanisms include mitochondrial dysfunction, oxidative stress and neuroinflammation. Ubiquinone is a fat-soluble vitamin found in the inner mitochondrial membrane and is involved in electron transport chain that supplies energy to vital organs. Resveratrol is a natural polyphenolic compound that has been shown to offer protective effects against many cardiovascular, neurodegenerative diseases and cancer. The aim of this work was to study the effect of the combination of L-dopa, ubiquinone and resveratrol in comparison with L-dopa alone on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced parkinsonism in mice. 50 albino mice were divided into 5 equal groups: Control untreated group, MPTP group, L-dopa + MPTP group, L-dopa + ubiquinone + MPTP group, L-dopa + resveratrol + MPTP group. Striatal dopamine, tumor necrosis factor alpha (TNF-α), glutathione reductase, malondialdehyde, nitric oxide, mitochondrial adenosine triphosphate (ATP), mitochondrial complex I activity and catalepsy score were measured. The combination between L-dopa and either ubiquinone or resveratrol induced significant increase in the striatal ATP, dopamine, glutathione reductase and mitochondrial complex I activity with significant decrease in striatal TNF-α level, nitric oxide and malondialdehyde level with significant improvement in the catalepsy score better than the group that received L-dopa alone compared to MPTP-treated group. In conclusion, the combination of L-dopa and ubiquinone or L-dopa and resveratrol had a better effect than L-dopa alone on MPTP-induced Parkinsonism in mice.