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Seroprevalence and Molecular Epidemiology of Brucellosis in Cattle in Egypt

Ahmed M El-Hady, Mohamed Sayed-Ahmed, Mohamed E Saleh and Emad E Younis

The aim of the present study was to evaluate of the Brucella vaccination program and immune response of vaccinated animals in Sharqyia Province. The study was applied on 710 lactating and non-lactating cows of some private farms and individual distributed animals on different districts in Sharqyia Province. Sera were collected from animals during routine diagnosis and control program. The results of screening tests Buffer acidified plate antigen test (BAPAT), Rose Bengal plate test (RBPT), Tube agglutination test (TAT), Complement fixation test (CFT), Rivanol test (RIVT) and indirect enzyme linked immunosorbent assay gave 124 and 176 seroreactive animals by incidence of 4.42% and 8.91% in private farms and individual cases respectively. 37 (29.8%) and 97 (55.1%) isolates of Brucella melitensis biovar 3 were recovered from 124 and 176 seroreactive animals respectively. In seroreactive cows, Brucella melitensis biovar 3 was isolated from 36% and PCR yielded expected products in 40%. In conclusion, more attention should be paid to the role of Brucella melitensis biovar 3 in brucellosis in cattle during the application of national program of Brucella control and eradication.