Jornal de Cromatografia e Técnicas de Separação
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Anion Exchange Chromatography for Purification of Antigen B of Cystic Echinococcosis

Jeyathilakan N, Abdul Basith S, John L, Chandran NDJ and Dhinakarraj G

Cystic echinococcosis or hydatidosis causes severe economic loss and public health problem to both human beings and livestock in many temperate and tropical areas of the world. Immuno diagnosis has been found to be useful not only in primary diagnosis but also for follow up of patients after surgical or pharmacological treatment in man. Various immune diagnostic tests for hydatidosis in man and animals have been attempted using hydatid cyst fluid antigens with varied sensitivity and specificity. However these assays using crude hydatid antigens have been non-specific due to cross reaction with Cysticercus, Coenurus and other helminthic infections. In order to overcome these difficulties various novel tests using purified antigens are essential for confirmative diagnosis of hydatidosis in man and animals. One of the subunit of antigen B, 8 kDa protein has been shown to be hydatid specific. Various types of chromatography have been used for purification of Hydatid antigens. However, Anion exchange chromatography had been used in this study to get more antigen B 8 kDa than other chromatographic methods.