Genômica e biologia de fungos
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Genome-Wide Identification and Characterization of the Laccase Family in Hypsizygus marmoreus and Expression Profile Analysis

Gang Wang, Rongcheng Liu, Hongbo Wang, Xiaoli Sun, Xiaoxiao Wu, Ying Zhu, Yuanyuan Wang, Yu chen, Lin Ma, Bobin Liu, Yuting Zheng, Fang Liu

Laccase exists widely in plants and fungi. It is a copper-containing polyphenol oxidase that can degrade lignin, oxidate and phenolic substances, inhibit heterophytes, promote fruiting body formation and improve the quality of mushrooms. In this study, 18 laccase genes were identified from the whole genome of a white strain of Hypsizygus marmoreus (HM62). These genes were mainly distributed on chromosomes 1, 2, 3, 4, 6, 9 and 10. Additionally, 9 genes were found to be clustered linearly on chromosome 6, indicating gene duplication. Multiple sequence alignment and protein similarity comparison revealed the presence of polymorphism among these laccase genes. Phylogenetic analysis showed that the laccase gene family of H. marmoreus was classified into four subfamilies. The spatiotemporal expression analysis of the laccase gene family showed that HmLac09 and HmLac10 were highly expressed in different periods and might be involved in lignin degradation and fruit body formation, respectively. The expression levels of HmLac02, HmLac05, HmLac08 and HmLac17 genes in gray or gray and white heterozygous strains were higher than those in white strains, which might be related to the difference in lignin decomposition in gray strains and one of the factors leading to different growth rates. The present study investigated the characterization of the H. marmoreus laccase gene family, extending our understanding of laccase mediated fruiting body development and growth rate mechanisms in this fungus.