Virologia e Micologia
Acesso livre

Abstrato

Genomic Analysis of Actinomycetes Obtained from Two Soda Lakes in Ethiopia

Kenesa Chali1*, Ketema Bacha2, Zerihun Belay1, Tolessa Muleta1

Background: As some previous studies suggested, regulatory elements in both promoter and gene body regions, Transcription Start Sites (TSSs) in the actinomycetes gene should be identified to understand gene regulatory mechanisms. Computational survey and in silico analysis was carried out for the actinomycetes genome which is isolated in two soda lakes of Ethiopia. Promoter regions and their regulatory elements were analyzed by using ten functional sequences.

Results: TSS for each of the ten (10) functional genes of isolated actinomycetes was identified. About 92% of actinomycetes genes contained more than two TSSs and a TSS with the highest predictive score was considered to determine a promoter region of the genes. Genes having more than one TSS and TSS of the highest prediction score was selected and investigated and our result showed that only actinomycete genes LC-21 from lake Chitu and LA-21 from lake Arenguade had a single TSS, whereas 92% of genes contained more than two TSSs. Generally, only the actinomycete isolate (LC-13) gene from Lake Chitu had a maximum of six (6) TSSs. A total of seven common candidate motifs were identified. The location and distribution of these candidate motifs in the promoter regions are dominated by Mot I relative to the TSSs. Depending on their significance value; alginate and motility regulator Z factor families (4.00e-01) were involved in the regulatory mechanism of actinomycetes genes. There is no CpG Island in both promoter and gene body regions of the actinomycetes genome.

Conclusion: Our investigation important to predict the functional gene of sequenced actinomycetes, novel biosynthetic gene clusters (inactivated or cryptic pathways) for secondary metabolite compounds from actinomycetes and opportunities for the biotechnology and pharmaceutical industries and further detail molecular characterization of the actinomycetes genome isolated from two soda lakes in Ethiopia.