Jornal de Bioquímica e Fisiologia Vegetal
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In Vitro Enhancement of Bacoside in Brahmi (Bacopa monnieri) Using Colchicine

Kharde AV, Chavan NS, Chandre MA, Autade RH and Khetmalas MB

Present investigation on in vitro effects of two levels of colchicine (0.1% and 0.2%) for 1, 2, 3, 4 and 5 hours treatment on leaf explants of Brahmi (Bacopa monnieri) collected from Paithan (Aurangabad) was conducted with a view to induce somaclonal variations and enhancement in the bacoside content in regenerated plants. Bramhi plants regenerated through previously treated leaf explants with colchicine showed morphological variations in leaf shape, number of leaves per node, leaf arrangement, etc. and enhance the bacoside content. 0.2% colchicine treatment for 5 hours showed maximum variations in number of leaves per node under in vitro conditions; however, these variations were not continued after subculture. While the 0.1% colchicine treatment for 2 hours significantly increase the bacoside content up to 0.72% i.e., more than four folds to the in vitro regenerated Brahmi plants without colchicine treatments.