Jornal de Bioquímica e Fisiologia Vegetal
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Abstrato

Using Heterologous Virus-Induced Gene Silencing in Ipomoea purpurea as a Sister Family of Solanceae

Tahereh Borna*

This study was conducted to investigate the possibility of using existing vectors as an heterologous system to silence genes in other plant species. In this study I measured the effectiveness of pre-existing Virus Induced Gene Silencing (VIGS) vector as a tool for gene silencing in Ipomoea purpurea, Ocimum basilicum and Anethum graveolens. The results showed that TRV-based VIGS vector with heterologous Phytoene Desaturase (PDS) sequences from Nicotiana bentamiana silenced the endogenous PDS gene in Ipomoea purpurea but not in Ocimum basilicum and Anethum graveolens.