Anestesia e Pesquisa Clínica
Acesso livre

Abstrato

The Effect of Dexmedetomidine and Propofol on Oxidative Stress and Antioxidizing System Studied on Liver Ischemia-reperfusion Model on Rats

Aykut Urfalıoglu, Mehmet Canturk, Erkan Yavuz Akcaboy and Nermin Gogus

Purpose: The presence or absence of protective or preventive role of dexmedetomidine and propofol against ischemia/reperfusion (I/R) injury at tissue level on liver I/R model was demonstrated.

Materials and methods: 24 rats were randomly divided into four groups. Following anesthesia laparotomy was done and hepatoduodenal ligament was explorated. Tissue samples were taken for both biochemical (MDA, SOD, GPx) and histological study.

Results: MDA level was hisghest in Group II and was significantly lower in Group III and Group IV. GPx level was lowest in Group II, the level of GPx was higher in Group III and Group IV with respect to Group II. SOD level was lowest in Group II and was highest in Group IV. SOD levels in Group II and Group III did not show statistical significance. In the histopathological study, in Group I the liver parenchyme and membrane integrity was protected; in Group II the hepatocyte cellular membrane integrity was distorted, microvesicule number increased and the liver sinusoids were shrunken; in Group III the hepatocyte membrane and nucleus was near normal and well protected; and in Group IV the cellular component structures were protected, hepatocyte cell membrane was protected in normal thickness.

Discussion: The results show that dexmedetomidine and propofol decrease the level of MDA in similarly; the effect of dexmedetomidine on GPx level was slightly lower than propofol and its effect on SOD level was lower than propofol. As a result, we believe that either propofol or dexmedetomidine can be effective in protecting hepatocytes from I/R injury especially in log term procedures but the former drug having dominance in this protective role.